Pseudofulvibacter gastropodicola sp. nov., isolated from a marine conch and emended descriptions of the genus Pseudofulvibacter Yoon et al. 2013 and Pseudofulvibacter geojedonensis.

A Gram-stain-negative, aerobic, rod-shaped and non-motile marine bacterium, designated MEBiC08749T, was isolated from a marine conch collected at Samcheok Port in the East Sea, Korea (also known as the Sea of Japan). 16S rRNA gene sequence analysis revealed that strain MEBiC08749T shared highest similarity with Pseudofulvibacter geojedonensis YCS-9T (94.9 %). Growth was observed at 5-31 °C (optimum, 25 °C), at pH 6.0-8.5 (optimum, pH 7.5) and with 0-6 % (w/v) NaCl (optimum, 2.5 %). The predominant cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 9 (comprising 10-methyl C16 : 0 and/or iso-C17 : 1ω9c). The DNA G+C content was 30 mol%. The only respiratory quinone was menaquinone-6 (MK-6). Phosphatidylethanolamine, two unidentified lipids and two unidentified aminolipids were detected as major polar lipids. Several phenotypic characteristics, such as production of acetoin, activity of cystine arylamidase, utilization of some carbohydrates, organic acids and amino acids, differentiated strain MEBiC08749T from P. geojedonensis KCTC 23884T. On the basis of data from this polyphasic taxonomic study, strain MEBiC08749T should be classified as representing a novel species in the genus Pseudofulvibacter, for which the name Pseudofulvibacter gastropodicola sp. nov. is proposed. The type strain is MEBiC08749T ( = KCCM 43046T = JCM 30369T). Emended descriptions of the genus PseudofulvibacterYoon et al. 2013 and of Pseudofulvibacter geojedonensis are also given.

Alcanivorax gelatiniphagus sp. nov., a marine bacterium isolated from tidal flat sediments enriched with crude oil.

A Gram-reaction-negative, rod-shaped marine bacterium, designated MEBiC08158(T), was isolated from sediments collected from Taean County, Korea, near the Hebei Spirit tanker oil spill accident. 16S rRNA gene sequence analysis revealed that strain MEBiC08158(T) was closely related to Alcanivorax marinus R8-12(T) (99.5% similarity) but was distinguishable from other members of the genus Alcanivorax (93.7-97.1%). The DNA-DNA hybridization value between strain MEBiC08158(T) and A. marinus R8-12(T) was 58.4%. Growth of strain MEBiC08158(T) was observed at 15-43 °C (optimum 37-40 °C), at pH 6.0-9.5 (optimum pH 7.0-8.0) and with 0.5-16% NaCl (optimum 1.5-3.0%). The dominant fatty acids were C16 : 0, C19 : 0 cyclo ω8c, C12 : 0, C18 : 1ω7c, C12 : 0 3-OH and summed feature 3 (comprising C15 : 0 2-OH and/or C16 : 1ω7c). Several phenotypic characteristics differentiate strain MEBiC08158(T) from phylogenetically close members of the genus Alcanivorax. Therefore, strain MEBiC08158(T) should be classified as representing a novel species of the genus Alcanivorax, for which the name Alcanivorax gelatiniphagus sp. nov. is proposed. The type strain is MEBiC08158(T) ( = KCCM 42990(T) = JCM 18425(T)).